ImageJ Macro files for the quantification of renal folic acid crystals from whole-slice tranverse sections imaged in brightfield without staining. One file automates the segmentation of the crystals and their quantification against the segmented total tissue. The other file generates a Euclidean distance map for the visualization and quantification of folic acid crystal spatial distribution in the kidney.
This repository accompanies:
A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury Ahmad Kamal Hamid, Eva Maria Pastor Arroyo, Sung Sik Lee, Carsten Alexander Wagner, and Daniela Egli-Spichtig. American Journal of Physiology-Renal Physiology 326(1): F105–F117, 2024.