diff --git a/test/js/bun/test/jest-doesnt-auto-import.js b/test/js/bun/test/jest-doesnt-auto-import.js
deleted file mode 100644
index 4d4a02b37378..000000000000
--- a/test/js/bun/test/jest-doesnt-auto-import.js
+++ /dev/null
@@ -1,12 +0,0 @@
-export function getJestGlobals() {
-  return {
-    describe: typeof describe === "function" ? describe : undefined,
-    it: typeof it === "function" ? it : undefined,
-    test: typeof test === "function" ? test : undefined,
-    expect: typeof expect === "function" ? expect : undefined,
-    beforeAll: typeof beforeAll === "function" ? beforeAll : undefined,
-    beforeEach: typeof beforeEach === "function" ? beforeEach : undefined,
-    afterAll: typeof afterAll === "function" ? afterAll : undefined,
-    afterEach: typeof afterEach === "function" ? afterEach : undefined,
-  };
-}
diff --git a/test/js/bun/test/test-auto-import-jest-globals.test.js b/test/js/bun/test/test-auto-import-jest-globals.test.js
index 5baeae43e917..c52605cb2830 100644
--- a/test/js/bun/test/test-auto-import-jest-globals.test.js
+++ b/test/js/bun/test/test-auto-import-jest-globals.test.js
@@ -1,3 +1,5 @@
+import { bunEnv, bunExe, tempDir } from "harness";
+
 test("Jest auto imports", () => {
   expect(true).toBe(true);
   expect(typeof describe).toBe("function");
@@ -10,15 +12,39 @@ test("Jest auto imports", () => {
   expect(typeof afterEach).toBe("function");
 });
 
-test("Jest's globals aren't available in every file", async () => {
-  const jestGlobals = await import("./jest-doesnt-auto-import.js");
+// Injection is a `bun test`-mode parser transform (#17734 made it apply to every
+// file loaded by the test runner, not just the entrypoint). The only remaining
+// scoping guarantee is that ordinary `bun <file>` / `bun -e` runs do NOT see it.
+describe("Jest globals are not injected outside of `bun test`", () => {
+  const names = ["describe", "it", "test", "expect", "beforeAll", "beforeEach", "afterAll", "afterEach"];
+  const source = `process.stdout.write(JSON.stringify({${names.map(n => `${n}: typeof ${n}`).join(", ")}}))`;
+  const expected = Object.fromEntries(names.map(n => [n, "undefined"]));
+
+  test.concurrent("bun -e", async () => {
+    await using proc = Bun.spawn({
+      cmd: [bunExe(), "-e", source],
+      env: bunEnv,
+      stdout: "pipe",
+      stderr: "pipe",
+    });
+    const [stdout, stderr, exitCode] = await Promise.all([proc.stdout.text(), proc.stderr.text(), proc.exited]);
+    expect(stderr).toBe("");
+    expect(JSON.parse(stdout)).toEqual(expected);
+    expect(exitCode).toBe(0);
+  });
 
-  expect(typeof jestGlobals.describe).toBe("undefined");
-  expect(typeof jestGlobals.it).toBe("undefined");
-  expect(typeof jestGlobals.test).toBe("undefined");
-  expect(typeof jestGlobals.expect).toBe("undefined");
-  expect(typeof jestGlobals.beforeAll).toBe("undefined");
-  expect(typeof jestGlobals.beforeEach).toBe("undefined");
-  expect(typeof jestGlobals.afterAll).toBe("undefined");
-  expect(typeof jestGlobals.afterEach).toBe("undefined");
+  test.concurrent("bun <file>", async () => {
+    using dir = tempDir("jest-globals-not-injected", { "entry.js": source });
+    await using proc = Bun.spawn({
+      cmd: [bunExe(), "entry.js"],
+      env: bunEnv,
+      cwd: String(dir),
+      stdout: "pipe",
+      stderr: "pipe",
+    });
+    const [stdout, stderr, exitCode] = await Promise.all([proc.stdout.text(), proc.stderr.text(), proc.exited]);
+    expect(stderr).toBe("");
+    expect(JSON.parse(stdout)).toEqual(expected);
+    expect(exitCode).toBe(0);
+  });
 });