diff --git a/src/runtime/webcore/Blob.rs b/src/runtime/webcore/Blob.rs
index 10c2ad3343f4..e12b0a0fd8cd 100644
--- a/src/runtime/webcore/Blob.rs
+++ b/src/runtime/webcore/Blob.rs
@@ -2064,13 +2064,27 @@ impl BlobExt for Blob {
         // index the full fixed-3 array (args[2] is written below regardless of len).
         let args = &mut arguments_.ptr[..];
 
-        if self.size.get() == 0 {
-            let ptr = Blob::new(Blob::init_empty(global_this));
-            // SAFETY: `ptr` just came from `heap::alloc` in `Blob::new`; force
-            // the inherent `Blob::to_js(&mut self)` over `JsClass::to_js`.
-            return Ok(unsafe { BlobExt::to_js(&*ptr, global_this) });
+        // The W3C relative-start/end clamp below needs the real size. For a
+        // lazy `Bun.file()` the size is still the `MAX_SIZE` sentinel, so
+        // negative `end` (and `start`) would be computed against that and
+        // over/under-read. Resolve now, same as `.size` does. A non-seekable
+        // file (pipe, FIFO) has no meaningful size; on macOS fstat reports the
+        // currently-buffered byte count there, which must not cap the slice.
+        if self.size.get() == MAX_SIZE && self.needs_to_read_file() {
+            self.resolve_size();
+            if let Some(store) = self.store.get() {
+                if let store::Data::File(file) = store.data_mut() {
+                    if file.seekable == Some(false) {
+                        self.size.set(MAX_SIZE);
+                    }
+                }
+            }
         }
 
+        // No `size == 0` early return: the clamp below already yields
+        // `(0, 0)` and `get_slice_from` keeps the store (so a missing file
+        // still surfaces ENOENT on read) and honours the `contentType` arg.
+
         // If the optional start parameter is not used as a parameter, let relativeStart be 0.
         let mut relative_start: i64 = 0;
         // If the optional end parameter is not used, let relativeEnd be size.
diff --git a/src/runtime/webcore/FileReader.rs b/src/runtime/webcore/FileReader.rs
index 2cb0fba6f932..41cedeb66e7d 100644
--- a/src/runtime/webcore/FileReader.rs
+++ b/src/runtime/webcore/FileReader.rs
@@ -604,19 +604,27 @@ impl FileReader {
         }
         let mut has_more = state != ReadState::Eof;
 
-        if !buf.is_empty() {
-            if let Some(max_size) = self.max_size {
-                let total_readed = self.total_readed.get();
-                if total_readed >= max_size {
-                    return false;
-                }
+        if let Some(max_size) = self.max_size {
+            let total_readed = self.total_readed.get();
+            if total_readed >= max_size {
+                // Cap already reached (includes `max_size == 0`). Windows
+                // delivers chunks async with `_buffer` still populated, and a
+                // later empty-buf/`on_reader_done` call would hand that
+                // over-read out. Clear it and close so nothing past the cap
+                // is ever delivered.
+                self.done.set(true);
+                self.reader().buffer().clear();
+                self.reader().close();
+                return false;
+            }
+            if !buf.is_empty() {
                 let len = (max_size - total_readed).min(buf.len());
                 if buf.len() > len {
                     buf = &buf[0..len];
                 }
                 self.total_readed.set(total_readed + len);
 
-                if buf.is_empty() {
+                if total_readed + len >= max_size {
                     close = true;
                     has_more = false;
                 }
diff --git a/test/js/bun/http/serve.test.ts b/test/js/bun/http/serve.test.ts
index 8f8d3de72e5b..7b0b35d1cf06 100644
--- a/test/js/bun/http/serve.test.ts
+++ b/test/js/bun/http/serve.test.ts
@@ -1673,7 +1673,6 @@ describe("should support Content-Range with Bun.file()", () => {
   const badRanges = [
     [10, NaN],
     [10, -Infinity],
-    [-(full.byteLength / 2) | 0, Infinity],
     [-(full.byteLength / 2) | 0, -Infinity],
     [full.byteLength + 100, full.byteLength],
     [full.byteLength + 100, full.byteLength + 100],
@@ -1691,6 +1690,17 @@ describe("should support Content-Range with Bun.file()", () => {
       });
     });
   }
+
+  // A negative start is a valid W3C relative index, not a bad range: slice(-n)
+  // addresses the last n bytes, same as ArrayBuffer.prototype.slice.
+  it(`negative start: ${-(full.byteLength / 2) | 0} - Infinity`, async () => {
+    const start = -(full.byteLength / 2) | 0;
+    await getServer(async server => {
+      const response = await fetch(`${server.url.origin}/?start=${start}&end=Infinity`);
+      expect(await response.arrayBuffer()).toEqual(full.buffer.slice(start, Infinity));
+      expect(response.status).toBe(206);
+    });
+  });
 });
 
 it("formats error responses correctly", async () => {
diff --git a/test/js/web/fetch/blob.test.ts b/test/js/web/fetch/blob.test.ts
index dc5ccbd1adf9..34db1ed9eb7b 100644
--- a/test/js/web/fetch/blob.test.ts
+++ b/test/js/web/fetch/blob.test.ts
@@ -27,12 +27,10 @@ for (const info of [
   {
     blob: new Blob(["Bun", "Foo"]),
     name: "Blob.slice",
-    is_file: false,
   },
   {
     blob: Bun.file(path.join(import.meta.dir, "fixtures", "slice.txt")),
     name: "Bun.file().slice",
-    is_file: true,
   },
 ]) {
   test(info.name, async () => {
@@ -45,11 +43,8 @@ for (const info of [
     expect(b2.size).toBe(0);
     const b3 = blob.slice(100, 3);
     expect(b3.size).toBe(0);
-    // file will lazy read until EOF if the size is wrong
-    if (!info.is_file) {
-      const b4 = blob.slice(0, 10);
-      expect(b4.size).toBe(blob.size);
-    }
+    const b4 = blob.slice(0, 10);
+    expect(b4.size).toBe(blob.size);
     expect(blob.slice().size).toBe(blob.size);
     expect(blob.slice(0).size).toBe(blob.size);
     expect(blob.slice(NaN).size).toBe(blob.size);
@@ -93,6 +88,81 @@ for (const info of [
   });
 }
 
+describe.concurrent("Bun.file().slice with relative start/end", () => {
+  // Each case slices a FRESH `Bun.file()` so the size has not been resolved by
+  // a prior `.size` read. The slice clamp must stat the file itself rather than
+  // compute against the lazy MAX_SIZE sentinel.
+  const cases: Array<[[number] | [number, number], string]> = [
+    [[1, -1], "12345678"],
+    [[0, -6], "0123"],
+    [[3, -3], "3456"],
+    [[0, -10], ""],
+    [[5, -5], ""],
+    [[0, -100], ""],
+    [[-3], "789"],
+    [[-3, -1], "78"],
+    [[-100], "0123456789"],
+    [[-100, -1], "012345678"],
+    [[0, 100], "0123456789"],
+    [[1, 3], "12"],
+  ];
+  const memBlob = new Blob(["0123456789"]);
+
+  test.each(cases)("slice(%p) size/text/bytes/arrayBuffer/stream", async (args, want) => {
+    using dir = tempDir("bun-file-slice-rel", { "f.bin": "0123456789" });
+    const p = path.join(String(dir), "f.bin");
+
+    // Each consumer reads a fresh slice of a fresh Bun.file().
+    const file = () => Bun.file(p).slice(...(args as [number, number]));
+
+    // In-memory Blob is the reference implementation.
+    expect({ size: want.length, text: want }).toEqual({
+      size: memBlob.slice(...(args as [number, number])).size,
+      text: await memBlob.slice(...(args as [number, number])).text(),
+    });
+
+    expect(file().size).toBe(want.length);
+    expect(await file().text()).toBe(want);
+    expect(Buffer.from(await file().bytes()).toString()).toBe(want);
+    expect(Buffer.from(await file().arrayBuffer()).toString()).toBe(want);
+    expect(await new Response(file().stream()).text()).toBe(want);
+  });
+
+  test("slice-of-slice with negative end", async () => {
+    using dir = tempDir("bun-file-slice-rel", { "f.bin": "0123456789" });
+    const p = path.join(String(dir), "f.bin");
+    const inner = Bun.file(p).slice(1, -1).slice(1, -1);
+    expect({ size: inner.size, text: await inner.text() }).toEqual({ size: 6, text: "234567" });
+  });
+
+  test(".size agrees with the bytes actually read", async () => {
+    using dir = tempDir("bun-file-slice-rel", { "f.bin": "0123456789" });
+    const p = path.join(String(dir), "f.bin");
+    const b = Bun.file(p).slice(1, -1);
+    const size = b.size;
+    const text = await b.text();
+    expect({ size, textLength: text.length }).toEqual({ size: 8, textLength: 8 });
+  });
+
+  test("slice of a nonexistent file still rejects with ENOENT on read", async () => {
+    using dir = tempDir("bun-file-slice-rel", {});
+    const bad = path.join(String(dir), "does-not-exist");
+    // Resolving the size for the clamp must not drop the File store; the
+    // read still has to surface the open() error, same as the unsliced case.
+    expect(async () => await Bun.file(bad).slice(0, 5).text()).toThrow(expect.objectContaining({ code: "ENOENT" }));
+    expect(async () => await Bun.file(bad).slice(1, -1).text()).toThrow(expect.objectContaining({ code: "ENOENT" }));
+  });
+
+  test("slice preserves the contentType arg when the source is empty", async () => {
+    using dir = tempDir("bun-file-slice-rel", { "empty.bin": "" });
+    const p = path.join(String(dir), "empty.bin");
+    // Same registry-normalised type a non-empty source produces.
+    const want = new Blob(["x"]).slice(0, 1, "text/html").type;
+    expect(Bun.file(p).slice(0, 5, "text/html").type).toBe(want);
+    expect(new Blob([]).slice(0, 0, "text/html").type).toBe(want);
+  });
+});
+
 test("new Blob", () => {
   var blob = new Blob(["Bun", "Foo"], { type: "text/foo" });
   expect(blob.size).toBe(6);