diff --git a/README.md b/README.md index 6696874..97afac4 100644 --- a/README.md +++ b/README.md @@ -47,8 +47,8 @@ Depending on the size of sequence set and on desired number of seeds to be analy Input files: - Necessary: - `rna_fastafile`: a fasta file with RNA sequences of interest - - `exp_values_file`: expression values in a csv format. It should have 2 or more columns, one column with names of of sequences, another column with values. The names of these two columns have to be specified with the `--anno_name_column` and `--measur_column` arguments. All the names of the sequences listed in this file must also have a corresponding sequence record in the fasta file provided with `rna_fastafile`. The measurement values might be integer or float numbers. - - `seeds_file`: a binary file containing the seeds to search through. Such file can be created with `seed_generator.py` script + - `exp_values_file`: expression values in a tsv format. It should have 2 or more columns, one column with names of of sequences, another column with values. The names of these two columns have to be specified with the `--anno_name_column` and `--measur_column` arguments. All the names of the sequences listed in this file must also have a corresponding sequence record in the fasta file provided with `rna_fastafile`. The measurement values might be integer or float numbers. + - `seeds_file`: a binary file containing the seeds to search through. Such file can be created with `seeds_generator.py` script - Optional: - user can include a file with RNA structure probing data (SHAPE or DMS-seq) to guide the possible match selection. There is no commonly used standard format for SHAPE RNA reactivity data; therefore, we are using the two-column SHAPE file format used by RNAstructure package ([link](https://rna.urmc.rochester.edu/Text/File_Formats.html#SHAPE)). SHAPE file provided by user should contain SHAPE profiles for multiple sequences, separated with `>`, like in fasta file. SHAPE file can be provided to the `filter_profiles_by_folding.py` script with the `--shape_profile` argument @@ -69,7 +69,7 @@ pyteiser_pipeline --rna_fastafile --exp_values_file